Protein Experiment, Protein Disulfide Bond, Reducing Reagent
Dithiothreitol
C
4H
10O
2S
2
M.W.: 154.25
99% Min
Melting Point: 41-42℃
For Molecular Biology Research Use
Soln. (5% in water): Clear and Colorless
Dithiothreitol (DTT) is, like beta-mercaptoethanol, a reducing reagent for proteins and protects the cysteine residues against oxidation.
Stock Conc.: 1M in ddH
2O, Store at -20℃
Working Conc.: 0.1-1mM
Cleland, W.W. (1964)
Biochemistry 3, 480-482
Dithiothreitol, a new protective reagent for SH groups.
Store at 4℃